T-SPOT. TB is a type of ELISPOT test used for the diagnosis of tuberculosis, which is included in the interferon gamma release test group. This test was made by Oxford Immunotec in England. It is available in most European countries, USA as well as various other countries. It was developed by researchers at Oxford University in England.
Video T-SPOT.TB
Test summary
T-SPOTÃ,®. TB calculates the number of antimicrobacterial effector T cells, white blood cells that produce interferon-gamma, in blood samples. This provides an overall measurement of the host immune response to mycobacteria, which may reveal the presence of infection with Mycobacterium tuberculosis, the tuberculosis-causing agent (TB). Because it does not depend on the production of reliable antibody response or recoverable pathogens, this technique can be used to detect latent tuberculosis. This technique has a relatively rapid advantage (results within 24 hours), and less affected by previous BCG vaccination compared with traditional testing methods for latent tuberculosis, the tuberculin skin test. This is due to the fact that both tests use different antigens for stimulation. While the tuberculin skin test uses a purified protein derivative, a heterogeneous mixture of more than two hundred different mycobacterial peptides, T-SPOT. TB using relative Mycobacterium tuberculosis -pecific antigen (peptides called ESAT-6 and CFP-10). ESAT-6 and CFP-10 are expressed by Mycobacterium tuberculosis , but none of all BCG vaccines are currently used and most of my mycobacteria are not translucent. Based on the principles of the test, it is estimated that T-SPOT. TB is more specific than the tuberculin skin test.
Maps T-SPOT.TB
Performance test
In one German study, 70 of 72 patients with confirmed TB infection were T-SPOT. TB positive, showing a sensitivity of 97.2%. However, recent data from studies on children with active tuberculosis in the UK show that the sensitivity of T-SPOT. TB may actually be worse than the tuberculin skin test (reported sensitivity is 66% and 82% respectively). The performance of interferon-gamma release tests in children has also been questioned by other publications. A meta-analysis of studies in children with active tuberculosis published in 2011 showed that T-SPOT sensitivity. TB is very similar to the tuberculin skin test (sensitivity collected is reported as 84% ​​and 80%, respectively).
References
External links
- T-SPOT. TB blood test for tuberculosis
- Oxford Immunotec Ltd.
Source of the article : Wikipedia
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